Modelo experimental de dieta rica em gordura é útil para avaliar o agravamento dos danos ao fígado associados a comorbidades

Ayane Araújo  Rodrigues; Even Herlany Pereira  Alves; Raissa Silva Bacelar de   Andrade; Luiz Felipe de Carvalho  França; Larissa dos Santos  Pessoa; Felipe Rodolfo Pereira da  Silva; David Di  Lenardo; Hélio Mateus Silva  Nascimento; André dos Santos  Carvalho; Francisca Beatriz de Melo  Sousa; Victor Brito Dantas  Martins; André Luiz dos Reis  Barbosa; Jand-Venes Rolim  Medeiros; Any Carolina Cardoso Guimarães  Vasconcelos; Daniel Fernando Pereira  Vasconcelos

doi:10.33448/rsd-v10i3.13012

Autores

Ayane Araújo Rodrigues Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0001-7792-6993
Even Herlany Pereira Alves Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0001-7566-1282
Raissa Silva Bacelar de Andrade Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0002-9126-3476
Luiz Felipe de Carvalho França Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0001-5361-8603
Larissa dos Santos Pessoa Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0001-6723-3069
Felipe Rodolfo Pereira da Silva Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0001-9224-5571
David Di Lenardo Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0002-4302-960X
Hélio Mateus Silva Nascimento Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0003-1551-8139
André dos Santos Carvalho Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0001-5259-6481
Francisca Beatriz de Melo Sousa Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0001-5265-2009
Victor Brito Dantas Martins Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0002-6932-915X
André Luiz dos Reis Barbosa Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0002-6452-9160
Jand-Venes Rolim Medeiros Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0002-7234-3382
Any Carolina Cardoso Guimarães Vasconcelos Instituto de Educação Superior do Vale do Parnaíba https://orcid.org/0000-0001-7235-5159
Daniel Fernando Pereira Vasconcelos Universidade Federal do Delta do Parnaíba https://orcid.org/0000-0002-3331-452X

DOI:

https://doi.org/10.33448/rsd-v10i3.13012

Palavras-chave:

Fígado gorduroso; Modelos animais; Dieta; Hepatopatia gordurosa não alcoólica.

Resumo

Objetivo: Desenvolver um modelo de dieta rica em gordura (DRG) que sozinho não resultou em doença hepática gordurosa não alcoólica (DHGNA), permitindo estudar a associação de comorbidades com o modelo de dieta rica em gordura. Material e métodos: Os ratos foram divididos em 2 grupos: dieta padrão e dieta rica em gordura, cada grupo com 8 animais. Os ratos foram submetidos à análise dos seguintes parâmetros no tecido hepático: dosagem de malondialdeído (MDA), glutationa (GSH) e atividade da mieloperoxidase (MPO). As amostras de fígado também foram avaliadas histopatologicamente quanto aos níveis séricos de alanina aminotransferase (ALT), aspartato aminotransferase (AST), albumina (ALB), fosfatase alcalina (FAL), ácido úrico e colesterol total (TC), cálcio (CA), ureia e HDL. Resultados: Mostraram que houve diferença significativa em MDA, GSH, colesterol total (CT), ALT, ALB, ácido úrico (AU), cálcio (CA) e HDL. A avaliação histopatológica apresentou escore baixo, insuficiente para a classificação da DHGNA. Conclusão: Este estudo demonstrou que o modelo de dieta rica em gordura não causou DHGNA. Esse achado permite utilizar a dieta hiperlipídica caracterizada neste estudo para investigar as possíveis alterações hepaticas causadas por outras comorbidades.

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Modelo experimental de dieta rica em gordura é útil para avaliar o agravamento dos danos ao fígado associados a comorbidades

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