Atividade da nisina contra Staphylococcus aureus resistente e sensível à meticilina e risco de aquisição de resistência
DOI:
https://doi.org/10.33448/rsd-v10i7.16178Palavras-chave:
Resistência a antibióticos; Atividade antimicrobiana; Bacteriocina; Staphylococcus aureus.Resumo
O objetivo deste estudo foi analisar o efeito antimicrobiano da nisina contra MRSA (Staphylococcus aureus resistente à meticilina) e MSSA (Staphylococcus aureus sensível à meticilina) e, ao mesmo tempo, examinar a possibilidade da bactéria desenvolver resistência à nisina. A susceptibilidade antimicrobiana das cepas foi testada pelos métodos de difusão em ágar e /ou microdiluição. Para selecionar cepas resistentes à nisina, as bactérias foram cultivadas consecutivamente em concentrações subletais da bacteriocina. A nisina apresentou atividade bactericida contra a maioria das cepas testadas. MRSA exigiu doses mais altas de bacteriocina em comparação com MSSA, tanto para inibição quanto para morte celular. No entanto, as transferências na presença de nisina podem eliminar completamente a atividade da nisina com um aumento no valor da concentração mínima inibitória de até 250 vezes. A resistência à nisina pode ser mantida em MRSA e MSSA mesmo na ausência da bacteriocina. A resistência à nisina afetou a suscetibilidade das linhagens principalmente aos antibióticos cefoxitina, oxacilina e eritromicina. Esses resultados indicam que a resistência à nisina é uma característica complexa entre MSSA e MRSA e deve ser elucidada antes da recomendação terapêutica da bacteriocina para o tratamento de infecções causadas por esse microrganismo.
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Copyright (c) 2021 César Matos Ribeiro da Silva; Waleska da Silva Albuquerque ; Jucyara Natállia Araujo de Oliveira ; Maria Regina Pires Carneiro ; Sona Jain ; Silvio Santana Dolabella ; Ana Andréa Teixeira Barbosa
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