Bioprospecção e caracterização de bactérias produtoras de celulase a partir do solo do Cerrado brasileiro

Autores

DOI:

https://doi.org/10.33448/rsd-v10i8.17426

Palavras-chave:

Screening; Enzima; Bacillus; Biotecnologia.

Resumo

Este trabalho descreve a bioprospecção e a caracterização de bactérias produtoras de celulase isoladas de solo obtido em uma região preservada do bioma Cerrado, em Uberaba, Brasil. A atividade celulolítica foi confirmada em 14 das 84 linhagens bacterianas isoladas. De acordo com a quantificação enzimática, cinco isolados foram selecionados como os melhores produtores de celulase e com base no sequenciamento parcial do gene 16S rRNA foram identificadas como Bacillus siamensis (isolado AB-9; 5.000U/mL), Bacillus toyonensis (isolado AB-1; 4.630U/mL), Bacillus methylotrophicus (isolados MB-3; 4.236U/mL e MP-7; 4.282U/mL) e Bacillus drentensis (isolado ME-2; 4.444U/mL). O extrato enzimático obtido de B. siamensis AB-9 foi o mais estável em diferentes valores de pH (2-8), mantendo sua atividade celulolítica, enquanto B. toyonensis AB-1 e B. methylotrophicus MP-7 produziram celulases com atividade máxima em pH 7 e 8. As celulases produzidas por B. siamensis AB-9 e B. toyonensis AB-1 apresentaram alta atividade enzimática em todas as temperaturas analisadas (10-80°C), enquanto as celulases de B. methylotrophicus MB-3 apresentaram atividade máxima na faixa de 20-70°C. Até o momento, esta é a primeira vez que bactérias produtoras de celulase isoladas do bioma Cerrado brasileiro na região do Triângulo Mineiro com potencial aplicação biotecnológica são descritas.

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Publicado

13/07/2021

Como Citar

ALVES, B. A. .; ROTTA, I. S. .; FERREIRA-MACHADO, A. B. .; PAIVA, A. D. Bioprospecção e caracterização de bactérias produtoras de celulase a partir do solo do Cerrado brasileiro. Research, Society and Development, [S. l.], v. 10, n. 8, p. e34010817426, 2021. DOI: 10.33448/rsd-v10i8.17426. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/17426. Acesso em: 23 nov. 2024.

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Seção

Ciências Agrárias e Biológicas