Expressão gênica de fatores imunológicos inflamatórios e parâmetros clínicos em pacientes com diabetes e não diabéticos com doença periodontal
DOI:
https://doi.org/10.33448/rsd-v11i1.24185Palavras-chave:
Diabetes mellitus; Doença Periodontal; Citocinas; Reação da Polimerase em Cadeia em Tempo Real.Resumo
Objetivo: Este estudo avaliou parâmetros clínicos, glicêmicos e imunológicos em pacientes com diabetes mellitus tipo 2 (DM) comparados àqueles sem alterações sistêmicas (NDM), ambos com periodontite crônica generalizada. Metodologia: Vinte e um pacientes foram selecionados com indicação de perda dentária. As cirurgias foram realizadas com retalho de Widman modificado para obtenção de colar gengival a 1 mm da margem gengival. Antes do procedimento cirúrgico, os seguintes parâmetros clínicos foram avaliados: profundidade de sondagem de bolsa (PPD), recessão gengival (GR), nível de inserção clínica (CAL) e sangramento à sondagem (BOP). Níveis de glicose em jejum (FGL) e hemoglobina glicosilada Hba1c (HbA1c) também foram avaliados. Durante a cirurgia, amostras de tecido gengival foram coletadas e congeladas para posterior análise laboratorial. As amostras foram processadas para obtenção de mRNA, cDNA e determinada a expressão gênica dos parâmetros imunológicos IL-1-β, IL-6, TNF-α, IL-10 e NF-kB por reação em cadeia da polimerase em tempo real (RT-PCR) Os dados foram analisados estatisticamente considerando p <0,05. Resultados: Os parâmetros clínicos e glicêmicos BOP, FGL e HbA1c foram estatisticamente maiores no grupo DM. Os níveis de RNAm de IL-1β, TNF-α e NF-kB foram maiores no grupo DM (p <0,05). Conclusão: A presença de diabetes e o estado hiperglicêmico aumentam os níveis de fatores imunes pró-inflamatórios e a gravidade da doença periodontal.
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Copyright (c) 2022 Gabriela Alessandra da Cruz Galhardo Camargo; Juliane Maria da Silva Rodrigues; Camila Silva de Amorim; Letícia de Farias Wenderoscky; Vinicius D’avila Bitencourt Pascoal; Amanda Caselato Andolfatto Souza; Cristiane Duque
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