Efeito do pH e da temperatura na produção e biomassa de fitase por fermentação submersa com Aspergillus niger var. phoenicis URM 4924
DOI:
https://doi.org/10.33448/rsd-v11i6.28994Palavras-chave:
Aspergillus niger; Ergosterol; Fitase; Fermentação submersa.Resumo
A produção de fitase e biomassa (estimada pelo teor de ergosterol) por fermentação submersa com Aspergillus niger var. phoenicis URM 4924 foi estudada. Ensaios experimentais foram realizados sob diferentes condições de pH (4,0 a 8,0) e temperatura (25 a 35 ºC), e a influência dessas variáveis nas respostas foi estudada por meio de um planejamento estatístico composto e metodologia de superfície de resposta. A produção de fitase e biomassa foi afetada pelo pH e temperatura utilizados durante a fermentação. A atividade da fitase aumentou em até 7,8 vezes (de 1,04 a 8,09 U/mL) e a concentração de ergosterol foi aumentada em até 38 vezes (de 9,3 a 354,09 µg/mL). Os valores máximos de ambas as respostas foram alcançados com pH 4,0 e 30 ºC. Boa correlação (ajuste de segunda ordem, R2 = 0,9875) foi encontrada entre os dados obtidos para atividade de fitase e teor de ergosterol, sugerindo que a produção de fitase é depende da formação de biomassa. Esses resultados são interessantes, pois contribuem para o desenvolvimento de um processo industrial de produção de fitase com elevados rendimentos por fermentação submersa.
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