Preliminary observations about the influence of the upstream homogenization pressure on the colloidal stability of UHT milk in the ethanol test

Authors

DOI:

https://doi.org/10.33448/rsd-v10i14.19411

Keywords:

Particle size; UHT processing; Ethanol.

Abstract

The purpose of this work was to study the influence of the upstream homogenization pressure (before heat treatment) on the stability of pasteurized whole milk when submitted to the alcohol test, aiming at UHT processing without the addition of stabilizers. Four different homogenization pressures (0 MPa, 20 MPa, 40 MPa and 80 MPa, in addition to the control treatment) were evaluated at a temperature of 80°C (average homogenization temperature used in the UHT milk industry). Two methods of observation of the colloidal behavior of milk after the alcohol test (ethanol concentrations between 72% (v/v) and 96% (v/v), at 2% (v/v) intervals) were applied: direct visualization and particle size distribution (LS) analysis. The LS results showed that the 0 MPa treatment did not present significant difference (p>0.05) for values of d10 up to 86% alcohol (v/v), thus showing a higher stability when compared to the control treatment (with stability up to 82% (v/v) alcohol). It was found that the stability of the control, 20 MPa and 40 MPa treatments were maintained up to 80% alcohol (v/v) (p>0.05); a distinct behavior was observed in the 80 MPa treatment which presented a statistically significant difference (p<0.05) after an alcohol concentration ≥78% (v/v). These data demonstrate that upstream homogenization applying pressures between 20 MPa and 80 MPa had a negative influence on the alcohol test and based on the results of the observations, the elimination of stabilizers could not be supported.

References

Akkerman, M., Johansen, L. B., Rauh, V., Sørensen, J., Larsen, L. B., & Poulsen, N. A. (2021). Relationship between casein micelle size, protein composition and stability of UHT milk. International Dairy Journal, 112, 104856. https://doi.org/10.1016/j.idairyj.2020.104856

Brasil. Ministério da Agricultura, Pecuária e Abastecimento. (1997). Regulamento técnico para fixação de identidade e qualidade do leite UHT (UAT). Diário Oficial da República Federativa do Brasil. Brasília.

Brasil. Ministério da Agricultura, Pecuária e Abastecimento. (2018). Instrução Normativa nº 76, de 26 de Novembro de 2018. Regulamento técnico de identidade e qualidade do leite cru refrigerado. Diário Oficial da União. Brasília.

Bylund, G. (2003). Dairy processing handbook. Serbian translation of handbook by Tetra Pak Processing System AB, Lund (Sweden).

Cano-Ruiz, M. E., & Richter, R. L. (1997). Effect of homogenization pressure on the milk fat globule membrane proteins. Journal of Dairy Science, 80, 2732e2739. https://doi.org/10.3168/jds.S0022-0302(97)76235-0

Costa, C. H. F. (2016). Avaliação Microestrutural do leite pasteurizado submetido ao teste do álcool visando processamento UHT. 103 f. Tese (Mestrado em Ciência e Tecnologia do Leite e Derivados) – Faculdade de Farmácia da Universidade Federal de Juiz de Fora, Minas Gerais.

Fox, P. F., Uniacke-Lowe, T., McSweeney, P. L. H., & O’Mahony, J. A. (2015). Heat-induced changes in milk. In Dairy chemistry and biochemistry (pp. 345-375). Springer, Cham.

Hansen, S. F., Petrat-Melin, B., Rasmussen, J. T., Larsen, L. B., Ostenfeld, M. S., & Wiking, L. (2018). Placing pasteurisation before or after microfiltration impacts the protein composition of milk fat globule membrane material. International Dairy Journal, 81, 35-41. https://doi.org/10.1016/j.idairyj.2017.12.015

Horne, D. S. (2016). Ethanol stability and milk composition. Advanced dairy chemistry, 225-246. https://doi.org/10.1007/978-1-4939-2800-2_9

Horne, D. S., & Parker, T. G. (1981). Factors affecting the ethanol stability of bovine milk: II. The origin of the pH transition. Journal of Dairy Research, 48(2), 285-291. https://doi.org/10.1017/S0022029900021701

Karlsson, M. A., Langton, M., Innings, F., Malmgren, B., Höjer, A., Wikström, M., & Lundh, Å. (2019). Changes in stability and shelf-life of ultra-high temperature treated milk during long term storage at different temperatures. Heliyon, 5(9), e02431. https://doi.org/10.1016/j.heliyon.2019.e02431

Karlsson, M. A., Langton, M., Innings, F., Wikström, M., & Lundh, Å. S. (2017). Variation in the composition and properties of Swedish raw milk for ultra-high-temperature processing. Journal of dairy science, 100(4), 2582-2590. https://doi.org/10.3168/jds.2016-12185

Lewis, M. J., & Heppell, N. J. (2000). Continuous thermal processing of foods. Aspen Publishers.

Lopez, C., Cauty, C., & Guyomarc’h, F. (2015). Organization of lipids in milks, infant milk formulas and various dairy products: role of technological processes and potential impacts. Dairy science & technology, 95(6), 863-893. https://doi.org/10.1007/s13594-015-0263-0

Michalski, M. C., & Januel, C. (2006). Does homogenization affect the human health properties of cow's milk?. Trends in Food Science & Technology, 17(8), 423-437. https://doi.org/10.1016/j.tifs.2006.02.004

Michalski, M. C., Michel, F., Sainmont, D., & Briard, V. (2002). Apparent ζ-potential as a tool to assess mechanical damages to the milk fat globule membrane. Colloids and Surfaces B: Biointerfaces, 23(1), 23-30. https://doi.org/10.1016/S0927-7765(01)00203-X

Obeid, S., Guyomarc’h, F., Francius, G., Guillemin, H., Wu, X., Pezennec, S., ... & Lopez, C. (2019). The surface properties of milk fat globules govern their interactions with the caseins: Role of homogenization and pH probed by AFM force spectroscopy. Colloids and Surfaces B: Biointerfaces, 182, 110363. https://doi.org/10.1016/j.colsurfb.2019.110363

Obeid, S., Guyomarc'h, F., Tanguy, G., Leconte, N., Rousseau, F., Dolivet, A., ... & Lopez, C. (2020). The adhesion of homogenized fat globules to proteins is increased by milk heat treatment and acidic pH: Quantitative insights provided by AFM force spectroscopy. Food Research International, 129, 108847. https://doi.org/10.1016/j.foodres.2019.108847

Sweetsur, A. M., & Muir, D. D. (1983). Effect of homogenization on the heat stability of milk. Journal of Dairy Research, 50(3), 291-300. https://doi.org/10.1017/S0022029900023128

Tetra Pak. (2021). Long-life milk. www.dairyprocessinghandbook.com.

Tsioulpas, A., Lewis, M. J., & Grandison, A. S. (2007). Effect of minerals on casein micelle stability of cows' milk. Journal of Dairy Research, 74(2), 167-173. https://doi.org/10.1017/S0022029906002330

Walstra, P., Walstra, P., Wouters, J. T., & Geurts, T. J. (2005). Dairy science and technology. CRC press.

Ye, A., Cui, J., Dalgleish, D., & Singh, H. (2017). Effect of homogenization and heat treatment on the behavior of protein and fat globules during gastric digestion of milk. Journal of dairy science, 100(1), 36-47. https://doi.org/10.3168/jds.2016-11764

Published

07/11/2021

How to Cite

CELESTINO, J. de A. .; STEPHANI, R.; WOLFSCHOON POMBO, A. F. .; CARVALHO, A. F. de; PERRONE, Ítalo T. . Preliminary observations about the influence of the upstream homogenization pressure on the colloidal stability of UHT milk in the ethanol test. Research, Society and Development, [S. l.], v. 10, n. 14, p. e396101419411, 2021. DOI: 10.33448/rsd-v10i14.19411. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/19411. Acesso em: 26 dec. 2024.

Issue

Section

Agrarian and Biological Sciences