Identification of the degradation of bread starch by saliva (salivary amylase enzyme) using Lugol 2%: an experimental proposal
DOI:
https://doi.org/10.33448/rsd-v11i11.27354Keywords:
Salivary amylase; Practical class; High school; Experimentation; Investigation; Human digestive system.Abstract
Animal Physiology is the area of Biology that is concerned with studying the functioning of the organism, however, it has many terms and processes, being a challenge for the full learning of students. Thus, nowadays, making the contextualization of this knowledge easier and more exciting is a constant challenge for the teacher. Added to this, classes based on the purely expository model can cause disinterest and attention deficit in students. To overcome such obstacles, the present work has as main objective, an experimental proposal, the students of the 2nd grade of High School, in the discipline of Biology, facilitating the contextualization of the Physiology of the human Digestive System, regarding the action of the salivary amylase enzyme (ptyalin) in bread starch, using Lugol 2%. As a result, students will realize that Lugol 2% will color the bread starch, with a very dark color, to the detriment of the complexation of the Iodine molecules of the said reagent, with the branched structure of the bread (polysaccharide). However, with the treatment that contains the piece of bread, Lugol 2% and saliva, the complexation of the Iodine molecules of the Lugol 2% reagent will not occur and its dark color will soon disappear, due to the fact that the administered saliva contains the enzyme Salivary amylase (ptyalin), which will break down the starch in bread (polysaccharide), turning it into disaccharide (maltose). Thus, it is expected that the involvement of students and discussions mediated by the teacher of the discipline, can facilitate the learning of the proposed theme, in addition to instigating students in the act of scientific experimentation. Finally, we can point out that the proposed experimental activity can be adapted to be carried out in the classroom, or at home by the students themselves, not requiring a physical laboratory structure, with glassware or expensive equipment.
References
Albuquerque, F. P., Miléo, J., De Lima, J. M. M., & Barbola, I. F. (2014). Entomologia no ensino médio técnico agrícola: Uma proposta de trabalho. Revista Eletrônica de Educação, 8(3), 251-265. http://www.reveduc.ufscar.br/index.php/reveduc/article/view/1030/370
Barreiros, A. L. B. S., & Barreiros, M. L. (2022). Aula 2 – Carboidratos Experimental. https://cesad.ufs.br/ORBI/public/uploadCatalago/11250927032012Quimica_Biomoleculas_Aula_02.pdf
Costa, B. B Fernandes, L. S., & De Paula, M. C. (2021). Ensino de matemática remoto: uma experiência inédita na educação básica. Revista Concilium, 21(1), 20-35, 2021. https://clium.org/index.php/edicoes/article/view/66/55
Estrela, C. (2018). Metodologia Científica: Ciência, Ensino, Pesquisa. Editora Artes Médicas.
Ferreira, G. L., Costa, V. C., & Araujo, M. H. (2008). Diminuição do amido em bananas maduras: um experimento simples para discutir ligações químicas e forças intermoleculares. Encontro Nacional de Ensino de Química [Internet]. Curitiba: XIV ENEQ. http://www.quimica.ufpr.br/eduquim/eneq2008/resumos/R0950-1.pdf
Gonçalves, T. M. (2021a). A guerra imunológica das células contra os patógenos: a proposta de um modelo didático tridimensional de baixo custo para simulação da resposta imune celular mediada por linfócitos TCD8+. Brazilian Journal of Development. 7(1), 4854-4860. https://doi.org/10.34117/bjdv7n1-329
Gonçalves, T. M. (2021b). Permeabilidade da membrana plasmática celular da beterraba: uma proposta de aula prática no ensino médio. Research, Society and Development. 10(3), 1-9. http://dx.doi.org/10.33448/rsd-v10i3.13479
Gonçalves, T. M. (2022). Identificando polissacarídeos vegetais: integrando naprática a Bioquímica e a Botânica no ensino médio. Revista ECCOM – Educação, Cultura e Comunicação. 13(25), 42-50. http://unifatea.com.br/seer3/index.php/ECCOM/article/view/1451/1593
Hill, R. W., Wyse, G. A., & Anserson M. (2012). Fisiologia Animal. (2ª ed.): Ed. Artmed.
Instituto Nacional de Estudos e Pesquisas Educacionais Anísio Teixeira, INEP (2019). Dados do censo escolar. https://www.gov.br/inep/pt-br/assuntos/noticias/censo-escolar/dados-do-censo-escolar--noventa-e-cinco-por-cento-das-escolas-de-ensino-medio-tem-acesso-a-internet-mas-apenas-44-tem-laboratorio-de-ciencias
Krasilchik, M. (2019). Prática de Ensino de Biologia. Edusp, São Paulo, 199p.
Loureiro, A. C., Sá, S. K. G., Nogueira, D. M., Comapa, S. S., Santos, B. M., Pereira, M. M., Souza, A. Q. L., & Nascimento, B, F, V. (2019). Estudo em alimentos cotidianos: Pesquisa de polissacarídeos através da reação com iodo. Brazilian Journal of Development, 5(11), 24243-24253. https://www.brazilianjournals.com/index.php/BRJD/article/view/4485
Lüdke, M., & André, M. E. D. A. (2013). Pesquisas em educação: uma abordagem qualitativa: E.P.U.
Murata, N. G., & Oliveira, A. L. (2017). Identificação e Análise de Atividades Investigativas em Livros Didáticos de Biologia. In XI Encontro Nacional de Pesquisa em Educação em Ciências – XI ENPEC Universidade Federal de Santa Catarina, Florianópolis, SC. http://www.abrapecnet.org.br/enpec/xi-enpec/anais/resumos/R0935-1.pdf
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. Santa Maria, 1° edição.
Reece, J. B., Urry, L. A., Cain, M. L., Wasserman, S. A., Minosrky, P. V., & Jackson, R. B. (2015). Biologia de Campbell. (10ª ed.): Artmed,
Sadava, D., Heller, H. C., Orians, G. H., Purves, W. K., & Hillis, D. M. (2009). Vida: a Ciência da Biologia Volume III: Plantas e Animais. 8ª ed, Porto Alegre: Ed. Artmed.
Sousa, T. O., Júnior, O. V. S., & Paixão, G. C. (2021). Ensino de Biologia: construção de conhecimento por meio de aulas práticas. RECH-Revista Ensino de Ciências e Humanidades –Cidadania, Diversidade e Bem Estar. 5(2), 443-468. https://periodicos.ufam.edu.br/index.php/rech/article/view/8975/6482
Tourinho e Silva, A. C., & Souza, D. N. (2020). Sequências de ensino investigativas para o ensino de ciências. Ed. CRV.
UFPB (2017). Laboratório Didático de Bioquímica. Teste do Iodo. http://plone.ufpb.br/ldb/contents/paginas/teste-do-iodo
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